On density of DNA

[Michael Cooley]

Antonio Rodriguez Franco (bb1rofra at lucano) wrote:
: I assume ethidium bromide increases the density of DNA. Is that is true,
: does anybody know what the density of DNA should be in the presence and in 
: the absence of EtBr ?.

: Antonio R. Franco
: Cordoba (Spain)


No. EtBr decreases the density of DNA. That is why the chromosomal DNA 
will band above the plasmid band in equilibrium centrifugation. The 
chromosomal DNA binds more EtBr due to it linear form.


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