Converting recA to rec+

Duncan Clark Duncan at
Mon Dec 5 05:14:53 EST 1994

Hi folks,

Does anyone have an easy way to change a recA1 E.coli (Invitrogen TOP10)
back to rec+. I know I can do P1 transduction (assuming I can find
something linked and selectable close to recA). Can I get revertants 
of recA1 ie if I UV irradiate will I select for revertants etc?

All help appreciated.


My mind's made up. Don't confuse me with the facts!

More information about the Methods mailing list