Converting recA to rec+

Duncan Clark Duncan at genesys.demon.co.uk
Mon Dec 5 05:14:53 EST 1994


Hi folks,

Does anyone have an easy way to change a recA1 E.coli (Invitrogen TOP10)
back to rec+. I know I can do P1 transduction (assuming I can find
something linked and selectable close to recA). Can I get revertants 
of recA1 ie if I UV irradiate will I select for revertants etc?

All help appreciated.

Duncan   

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