plasmid sub-genomic libraries

Wayne Gottlieb wig1 at
Tue Dec 6 13:24:57 EST 1994

Does anyone have experience screening subgenomic plasmid libraries?  We are
having trouble seeing clear colonies.  Some lifts come out clear, but most
are very fuzzy and/or dim.  we think we have narrowed it down to the actual
lift and pretreatment.  How critical are pretreatment conditions?  Thanks

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