Lambda DASH DNA isolation

Sasha Kraev bckraev at aeolus.ethz.ch
Tue Dec 6 13:19:01 EST 1994


In my hands the procedure from CPMB Manual (1.13.7) for liquid lysates worked
well with the host SRB, supplied by Stratagene with DASH libraries.
However, it is true that some clones give yield much lower than usual, for
those one has to optimize phage to cell ratio on infection, even then they
may not propagate efficiently. Hope this helps.



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