Help me on RNA sequencing
shzhang at uoguelph.ca
Tue Dec 6 21:51:38 EST 1994
Hi. You can check your viral RNA to see how pure it is. I did lots of
direct viral RNA sequencing and it was not problem to read.
On Wed, 7 Dec 1994, Joan Rowe wrote:
> : It's me again. I need help on RNA sequencing. When I sequenced viral RNA,
> : the result was so many ladders on all 4 lanes.
> I did a lot of direct virus RNA sequencing a few years back and always
> had trouble with bands across all four lanes until I started adding a
> terminal transferase chase reaction. It works like a charm (most of the
> time). But if your primer is priming several different viruses, you will
> not get clean sequence even then. You have to be sure that the primer is
> specific ony for the virus of interest.
> If you want the method I can dig it out, but don't have it at hand right now.
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