Help me on RNA sequencing

[Shula Zhang]

Hi. You can check your viral RNA to see how pure it is. I did lots of 
direct viral RNA sequencing and it was not problem to read.
Shulu Zhang


On Wed, 7 Dec 1994, Joan Rowe wrote:

> 
> : It's me again. I need help on RNA sequencing. When I sequenced viral RNA,
> : the result was so many ladders on all 4 lanes.
> 
> Hi,
> 
> I did a lot of direct virus RNA sequencing a few years back and always 
> had trouble with bands across all four lanes until I started adding a 
> terminal transferase chase reaction.  It works like a charm (most of the 
> time).  But if your primer is priming several different viruses, you will 
> not get clean sequence even then.  You have to be sure that the primer is 
> specific ony for the virus of interest.
> 
> If you want the method I can dig it out, but don't have it at hand right now.
> 
> Joan
> 
>