DNA from old blood samples??

Chris Troy cstroy at mail.tcd.ie
Tue Dec 6 06:18:27 EST 1994


Does anyone have any suggestions or protocols for extracting DNA from some
old blood samples that have been lying around for up to two years. The
samples are feline bloods that were collected in lithium heparin vacutainer
tubes and stored at 4C in a refrigerator. The blood hasn't quite coagulated
yet, but its very gloopy and definitely not liquid. We are reluctant to try
our normal protocol as these samples are irreplaceable. The quality of the
DNA is not necessarily a factor because they shall only be used for PCR.

Thanks in advance,


Chris Troy,           E-mail: cstroy at mail.tcd.ie
Genetics Department,     Phone:  (353)-1-7021265
Trinity College,         Fax:    (353)-1-6798558
Dublin 2.

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