request help with immunofluorescence!

[BARNETT.YA]

I need help to detect p53 in MELC cell lines using mAB 421 
and FITC-conjugates..I cannot detect any fluorescence
in the control cells (C127I) which are known to contain
wild-type p53..anybody can help?

A. Dallol
Sch. Biomed. Sc.
UUC
Colerina
(sorry..Coleraine)
BT52 1SA N. Ireland
phone : +44 1265 44141 ext 4730
fax: +44 1265 40906
email: BTS815 at uk.ac.ulst.ucvax