request help with immunofluorescence!

BARNETT.YA BTS815 at UCVAX.ULSTER.AC.UK
Tue Dec 6 16:22:47 EST 1994


I need help to detect p53 in MELC cell lines using mAB 421 
and FITC-conjugates..I cannot detect any fluorescence
in the control cells (C127I) which are known to contain
wild-type p53..anybody can help?

A. Dallol
Sch. Biomed. Sc.
UUC
Colerina
(sorry..Coleraine)
BT52 1SA N. Ireland
phone : +44 1265 44141 ext 4730
fax: +44 1265 40906
email: BTS815 at uk.ac.ulst.ucvax



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