beads for transformation

Cornelius Krasel krasel at
Tue Dec 6 14:23:57 EST 1994

Dr Tay Hou Ngee Agnes (phstayhn at wrote:
> I recall having used beads for spreading bugs when doing transformations 
> (instead of using pasteur pipets bent into spreaders).

There are lots of methods to spread Colis. You can use a spreader (also
called Drigalski spatula), you can use ordinary glass beads, you can
use glass pipets, you can just add a lot of Colis and let them dry
(although the latter method is not recommended by Maniatis et al.).
Anything to add?


/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany  */
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