Blotter-comparison

Stephen R. Lasky, Ph.D. Stephen_Lasky at brown.edu
Tue Dec 6 15:00:14 EST 1994


In article <3bqbph$7et at mserv1.dl.ac.uk>, christian a geltinger
<geltinger at gsf.de> wrote:

> Dear netters,
> 
> unexpectedly at the end of the year we have some money left (you believe that 
> ?). So we decided to buy a blotter.
>  Does somebody have experiences either bad or good with electro and vacuum 
> blotters to do southern blots mainly? Fragments to resolve will be in
the range 
> of up to 25 kb. Any recomendations and tips will be highly appreciated. 
> 
> Please respond directly to the e-mail address
> 
> uj23116 at sunmail.lrz-muenchen.de
> 
> not to the newsgroup itself ! (We haven+t subscribed yet)
> 
> Dr. Horst Schmieger
> Institut fuer Genetik der LMU Muenchen
> Tel.: ++49/89/ 1791 9816
> Fax.: ++49/89/ 1791 9820
> e-mail: uj23116 at sunmail.lrz-muenchen.de

The vacuum blotters that I have used leave a lot to be desire. 
Electroblotters are convenient in that they can also be used for western
blotting, but they use a lot of reagents, require a power suppy and are
expensive.  Positive Pressure blotters (Posiblotters) give better
transfers than vacuum blotters (IMHO).  Since one normally does (or at
least I always do when I want to transfer large DNA's) a limited acid
hydrolysis of DNA that is to be transfered, the size of your DNA is not
really important.

These are just my opinions.

SRLasky

-- 
*********************************************************************
Stephen R. Lasky, Ph.D.
Roger Williams Medical Center/Brown University
Phone: 401-456-6572       Fax: 401-456-6569       e-mail: Stephen_Lasky at brown.edu
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
"To me at least, 'Yuck' doesn't capture the full essence of death by
neurotoxin."   -Dick Dunn
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