SDS-PAGE gel problem
Kitty Kit Chui
ez027154 at dale.ucdavis.edu
Tue Dec 6 22:40:37 EST 1994
In the past, other people in my lab were able to resolve the
phosphorylated and unphosphorylated forms of the protein in SDS-PAGE gel
with 5% for running and 3% for stacking. The ratio of acrylamide : bis
is 30%-0.8%. The phosphorylated form usually runs at a much higher
molecular weight than the unphosphorylated one. However, my gel kept
giving only one band. I have even tried other solutions in the lab made
by other people and it gave the same results. I even included a marker,
a mixture of the two forms, and it failed to resolved too.
The bands seem to be OK; they are sharp flat bands. (The MW is about 200kD)
However, the problem is I cannot resolve the two forms.
If anyone has any idea what I should check in my experiment, please e-mail me
(kkchui at dale.ucdavis.edu) or reply here.
Thank you for your help in advance.
Section of Molecular Biology and Biochemistry
More information about the Methods