SDS-PAGE gel problem

Kitty Kit Chui ez027154 at dale.ucdavis.edu
Tue Dec 6 22:40:37 EST 1994


In the past, other people in my lab were able to resolve the 
phosphorylated and unphosphorylated forms of the protein in SDS-PAGE gel 
with 5% for running and 3% for stacking.  The ratio of acrylamide : bis 
is 30%-0.8%.  The phosphorylated form usually runs at a much higher 
molecular weight than the unphosphorylated one.  However, my gel kept 
giving only one band.  I have even tried other solutions in the lab made
by other people and it gave the same results.  I even included a marker, 
a mixture of the two forms, and it failed to resolved too.  

The bands seem to be OK; they are sharp flat bands.  (The MW is about 200kD)
However, the problem is I cannot resolve the two forms.

If anyone has any idea what I should check in my experiment, please e-mail me 
(kkchui at dale.ucdavis.edu) or reply here.
Thank you for your help in advance.

Kitty Chui
Section of Molecular Biology and Biochemistry
UC Davis



More information about the Methods mailing list