Total RNA vs mRNA for diff. display

Mike Rott mrott at unixg.ubc.ca
Wed Dec 7 14:11:58 EST 1994


 
> quote:
> Why  does total RNA yield better results than mRNA in the mRNA
> differential display technique.
> endquote.

I think one of the potential problems with using purified mRNA for
differential display is that some contamination of your purified mRNA with
oligo dT is possible.  This could could lead to a laddering effect as
opposed to a distinct banding pattern.  Haven't confirmed this myself
though.

Mike



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