Primer selection

Michael Gorry mcgorry at med.pitt.edu
Thu Dec 8 16:57:03 EST 1994


Can anyone indicate a source that can be used to obtain the information
needed when selecting primers?
What I mean, is there any written explanation of what selection parameters
(Tm, primer-dimer formation, hairpin
loops, and the energy values associated with these, salt concentrations,
primer concentrations, etc) to use as cutoffs
when selecting a possible primer.  While there is a lot of information
available on PCR itself (reaction optimization,
cycling conditions, etc.) there is very little available on the
recommended selection parameters to use when
selecting the primers.  Any help will be appreciated.

Thanks

-- 
Michael Gorry
mcgorry at med.pitt.edu



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