Primer selection

Tim Hutchin hutchin at hsc.usc.edu
Thu Dec 8 11:31:54 EST 1994


In article <mcgorry-0812941658450001 at quadra950.pathology.pitt.edu>,
mcgorry at med.pitt.edu (Michael Gorry) wrote:

> Can anyone indicate a source that can be used to obtain the information
> needed when selecting primers?

   You could try using the Oligo primer analysis software put out by
National Biosciences.  I find it works very well for showing primer duplex
formation, hairpins and other such secondary structures and all the others
things you mentioned. Though it does recomend ridiculous annealing
temperatures and its not that great at selecting primers for a given DNA
sequence. Its biggest drawback? It costs a couple of hundred $.



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