Isolation of Nuclei

In C. Kim inkim at
Thu Dec 8 17:53:57 EST 1994

Does anyone have any experience in isolating nuclei from human leucoytes
(or any cell line)?  Since we are interested in doing in situ 
hybridization, we would like to avoid including any detergent during the
isolation process.  Detergent will destroy nuclear structure.  Any 
protocol will be greatly appreciated.  Thank you.

In C. Kim   <inkim at>    
University of New Mexico School of Medicine
Albuquerque, New Mexico  

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