Westerns without blocking step?

Tracy Aquilla aquilla at salus.med.uvm.edu
Thu Dec 8 11:40:19 EST 1994


In Article <3c44pi$f86 at dux.dundee.ac.uk>, hjtreuma at dux.dundee.ac.uk (H.J.
Treumann Biochemistry ext 4301) wrote:
>Dear Gary, 
>
>what is FBS?
>
>Achim Treumann
>a.treumann at dundee.ac.uk

Fetal Bovine Serum, very expensive stuff.

>
>Gary Butler (butler at soma.UMDNJ.EDU) wrote:
>
>[... some good advice omitted ...]
>
>: The best 
>: blocking agent for excellent signal and excellent signal to noise ratio 
>: for me is 10% FBS, 0.05% w/v tween-20, 100 U/ml penn G.  Block for a minimum
>:
>: Gary H. Butler, Ph.D.
>: Coriell Institute for Medical Research
>: 401 Haddon Ave.
>: Camden, NJ 08103
>
>: Tel: (609) 757-9716
>: Fax: (609) 964 0254 Attn: Butler
>: Email: butler at UMDNJ.edu



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