Help with Lambda DNA isolation
Peter Emanuel
pemanuel at umabnet.ab.umd.edu
Thu Dec 8 10:49:12 EST 1994
In article <pabkwc.2.000F4A21 at leeds.ac.uk>, pabkwc at leeds.ac.uk (K.W.
Cheung) wrote:
> Can someone recommend a good method for Lamda DNA isolation. I've tried
using
> the Qiagen midi prep using plate lysate but only got small amount of DNA
back.
> What is the best host for the propagation of Lamda DASH II ?
>
> Thanks.
I have used the wizard lambda kit with good results. The yeild for 10 mls
is around 6 ugs and it is undegraded. Do the optional proteinase K step.
Peter Emanuel
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