Help with Lambda DNA isolation

Peter Emanuel pemanuel at umabnet.ab.umd.edu
Thu Dec 8 10:49:12 EST 1994


In article <pabkwc.2.000F4A21 at leeds.ac.uk>, pabkwc at leeds.ac.uk (K.W.
Cheung) wrote:

> Can someone recommend a good method for Lamda DNA isolation.  I've tried
using 
> the Qiagen midi prep using plate lysate but only got small amount of DNA
back. 
>  What is the best host for the propagation of Lamda DASH II ?
> 
> Thanks.
I have used the wizard lambda kit with good results.  The yeild for 10 mls
is around 6 ugs and it is undegraded.  Do the optional proteinase K step.
   Peter Emanuel



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