Cell wall degradation desired..

[David R. Rockhold]

How aout a french press?
>I am working with a Gram + bacterium (Arthrobacter nicotinovorans). Part 
>of my experiments involve detection and purification of DNA binding 
>proteins present on cell extracts of this bug. I use to prepare my cell 
>extracts with a sonifier, but this procedure is very unefficient. 
>I added enzymes (lysozyme and/or mutanolysin, both from Sigma) prior
> to the sonification in order to try to improve the lysis efficiency,
> but it seems it did not help. 
>
>Does anybody has ideas which could help me improving my process?
>Does anybody knows of an enzyme attacking efficiently Gram + bacteria?
>
>Thanks in advance,
>
>Castor.
>
>menendez at mibm.ruf.uni-freiburg.de
>
>
Dave Rockhold, Microbiologist          Phone 510-559-5722
800 Buchanan Street                    Fax   510-559-5775
Albany, CA  94710