Cell wall degradation desired..
David R. Rockhold
drr at PW.USDA.GOV
Fri Dec 9 18:40:29 EST 1994
How aout a french press?
>I am working with a Gram + bacterium (Arthrobacter nicotinovorans). Part
>of my experiments involve detection and purification of DNA binding
>proteins present on cell extracts of this bug. I use to prepare my cell
>extracts with a sonifier, but this procedure is very unefficient.
>I added enzymes (lysozyme and/or mutanolysin, both from Sigma) prior
> to the sonification in order to try to improve the lysis efficiency,
> but it seems it did not help.
>Does anybody has ideas which could help me improving my process?
>Does anybody knows of an enzyme attacking efficiently Gram + bacteria?
>Thanks in advance,
>menendez at mibm.ruf.uni-freiburg.de
Dave Rockhold, Microbiologist Phone 510-559-5722
800 Buchanan Street Fax 510-559-5775
Albany, CA 94710
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