Reverse transcription and RNA second. structure

[Stephen R. Lasky, Ph.D.]

In article <krzychwl-091294134232 at hygeia.nidr.nih.gov>,
krzychwl at helix.nih.gov (krzych) wrote:

> Can reverse transcriptase jump over a loop of secondary structure and
> transcribe further, yelding shorter transcripts?

Yes.  It can also loop back around and give you a duplication within a
transcript.

> Is there any published documentation of this phenomenon?

I don't know about documentation, but we published a paper in 1987 that
had an incorrect sequence of IGF-1 because of this type of artifact in a
rat liver cDNA library from Clonetech. We published it in MoleEndo and
submitted it to GenBank.  I'm sure its corrected by now (I'm not working
in that field anymore).

SRLasky

-- 
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Stephen R. Lasky, Ph.D.
Roger Williams Medical Center/Brown University
Phone: 401-456-6572       Fax: 401-456-6569       e-mail: Stephen_Lasky at brown.edu
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"To me at least, 'Yuck' doesn't capture the full essence of death by
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