ssDNA templates by i primer PCR

Gareth Gareth
Fri Dec 9 12:32:32 EST 1994


I have recently seen a protocol for producing ssDNA templates
for sequencing by performing a PCR reaction on 2-10% of a PCR
product using only one primer. I have successfully amplified 
a ssDNA template this way but have not yet sequenced it. Has
anyone out there been successful with this technique, if so
is there anything I should know before starting?
Thanks
Gareth



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