Autofluorescence cures?

Corrie Allen szcorrie at peseta.ucdavis.edu
Sat Dec 10 10:07:06 EST 1994


I'm trying to perform fluorescent in-situ hybridization on fixed neonatal
lung tissue and I've run into a serious roadblock.  The lungs
autofluoresce brighter than my probe does.  Even unfixed, frozen lung
fluoresces.  I was wondering if someone might have run into this before
and figured out a way to eliminate the tissue autofluorescence without
destroying tissue morphology or wiping out the target mRNA. 



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