HPLC capability question.

BSS169 at BANGOR.AC.UK BSS169 at BANGOR.AC.UK
Sun Dec 11 10:39:57 EST 1994


In a massage, someone asked IF IT'S POSSIBLE TO USE HPLC INSTEAD OF ELISA TO
QUANTIFY TWO PROTEINS IN A SUSPENSION.

Theoretically saying, Yes, it's possible. If (1) you can separate the two 
proteins from other proteins (if any) in the suspension, (2) separation results are constant, and (3) you have already got the two proteins in high purity as
standards.

But I haven't seen any reason why you want to use HPLC in stead of ELISA. You 
have been using ELISA which has advantages over HPLC in specificity, sensi-
tivity, efficiency and costs for this task.

If somebody asks me to do this job by ELISA for $1, or by HPLC for $50, I will 
definitely choose the first one.

Dan Wu

School of Biol. Sci.,
Univ. of Wales,
Bangor,
UK

E-mail:bss169 at bangor.ac.uk



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