373 Stretch (PE/ABI): Sequenase Kits

G. Dorado .bb1dopeg at lucano.uco.es
Sun Dec 11 05:27:00 EST 1994


Has anyone tried the new Sequenase kits for the 373 Stretch sequencer
from Applied Biosystems (PerkinElmer) including:

--PRISM Sequenase Terminator Single-Stranded DNA Sequencing Kit
--PRISM Sequenase Terminator Double-Stranded DNA Sequencing Kit

Does the T7 Gene 6 Exonuclease step really work to get single stranded
DNA from dsPCR products? How much DNA should be used (µg, please).

Thanks,

G. Dorado
Internet Email: bb1dopeg at lucano.uco.es



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