to DNA sequencing experts
bckraev at aeolus.ethz.ch
Sun Dec 11 22:40:13 EST 1994
There is something in the way your co-worker handles the DNA polymerase
or something is wrong with his pipet, so that a variable amount of
polymerase activity gets into the samples. Multiple bands across all four tracks
are a sure sign of this. In addition, he may be sequencing a very large DNA,
that when dissolved, gives a viscous solution. This needs quite some effort
for mixing in the polymerase and possibly other components.
To locate the problem, it is best to spend one day sequencing 4 or 5
aliquots of empty vector prep and see how they come out.
Hope this helps. Sasha
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