RT reagents affect PCR

Sean David Moore smoore at mail1.sas.upenn.edu
Mon Dec 12 10:46:11 EST 1994





ANY help on this matter would be appreciated....I can say this.

In our RT-PCR RxN's...we can get outstanding results...sometimes...

If the reagents become more than a week or two old...something inhibits 
the PCR step...

I know you say contamination etc...this has been a question for some 
time...it is not the problem.  Aside from the tips used...everything was 
checked.  If we include  a DNA-based PCR
...something inhibits the PCR step.

These are Random-Primed as well.

RSVP.

Sean





More information about the Methods mailing list