RT reagents affect PCR
Sean David Moore
smoore at mail1.sas.upenn.edu
Mon Dec 12 10:46:11 EST 1994
ANY help on this matter would be appreciated....I can say this.
In our RT-PCR RxN's...we can get outstanding results...sometimes...
If the reagents become more than a week or two old...something inhibits
the PCR step...
I know you say contamination etc...this has been a question for some
time...it is not the problem. Aside from the tips used...everything was
checked. If we include a DNA-based PCR
...something inhibits the PCR step.
These are Random-Primed as well.
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