question about yeast GAL4 DNA binding sequence

[PRAMATHESH PATEL]

Hi:
    I have the following two questions regarding the GAL4 DNA binding
sequence:

1)  Is the following 18mer sequence the most optimal DNA binding sequence
for GAL4:

5'---CGGAAGACTCTCCTCCG---3'

and

2)  If I were to make a synthetic binding site, and would like to arrange
two binding sites in tandem, what is the optimal distance (# of bp) between
the two sites.

Thanks in advance,

Prem



e-mail  Patel_P at BMS.com