protein expression problem (maybe SS bonds)

HARDIES at THORIN.UTHSCSA.EDU HARDIES at THORIN.UTHSCSA.EDU
Mon Dec 12 20:43:36 EST 1994


Mungo at uvvm.uvic.ca wrote:

> ... Are there any problems associated with expressing extra-
cellular [proteins in the cytoplasm of] bacteria.  ...

Extracellular proteins often have disulfide bonds which won't form in the 
reducing environment in the coli cytoplasm.  You might look for your
protein in inclusion bodies, by solubilizing them.  Alternatively,
you may have to supply a bacterial signal sequence.

Hope this helps.
Steve Hardies, Dept. of Biochem., Univ. of Texas HSC at San Antonio
Hardies at thorin.uthscsa.edu





More information about the Methods mailing list