artifact in stratagene lambda zap libraries

Rob Steele resteele at uci.edu
Tue Dec 13 22:16:09 EST 1994


In article <-0812942020030001 at b151-mac.stanford.edu>, (Tuba Buddha
Acolyte) wrote:

> Recently I've screened two different rat libraries from Stratagene, a
> brainstem/spinal cord and an adult whole brain.  After sequencing several
> different clones, I realized that all of my clones have the same 8
> nucleotides after the RI site, GGCACGAG, and then the clones are
> different.  Is this a artifact of attaching the RI linkers?
> 
> Please e-mail responses to "ting at cmgm.stanford.edu"


Stratagene uses an adapter to put the EcoRI sites on the ends of the
cDNAs.  The sequence you see is the rest of the adapter.  The whole
adapter sequence is GAATTCGGCACGAG.

-- 
Rob Steele
Department of Biological Chemistry
University of California, Irvine
Irvine, CA 92717-1700



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