Sau96I doesn't cut well

Claudia Benkwitz UZS13B at ibm.rhrz.uni-bonn.de
Tue Dec 13 20:53:10 EST 1994


Dear colleagues
 
I have problems  with the enzyme Sau96I(Recognition sequence: GGNCC).
I already varied the units up to 80U/500ng and additionally used different
buffers(SIGMA/Boehringer) but that didn't change anything.An incubation
time of 40 hours didn't improve cutting,too.
Who can give me advice or can give me the address of a company
selling an isoschizomer?
 
I will be very thankful for any help.
 
 Claudia Benkwitz
 Medical Department of the University of Bonn
--
Using Internet-Account of Stefan Kahlert



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