Protein expression

bipin dalmia dalmiabk at phibred.com
Tue Dec 13 17:19:30 EST 1994


In article <941212.151331.PST.MUNGO at UVVM.UVic.CA> , MUNGO at UVVM.UVIC.CA
writes:
>WE are trying to express some ECM receptor cDNAs in E coli and are having
>no luck.  The cDNA being expressed contains no signal peptide, or
transmembrane
>domains, and is not being expressed as a fusion protein.  Upon induction
we
>get nothing.  The control plasmid works well under the same
circumstances.  The
>insert is in the right frame and orientation.  Does anyone have any
suggestions
>as to what I can do ? Are there any problems associated with expressing
extra-
>cellular molecules in bacteria.  A quick review of the literature
reveals that
>most antibodies are raised against synthetic peptides.  Is this
significant ?
>Any help appreciated as with no expression it is hard to decide what to
change.

what expression vector are you using? how do your cells grow _after_
induction? some proteins may be lethal to the bugs. 

bip



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