Help with sequencing

Geoff Goellner goelg at newton.mayo.edu
Mon Dec 12 20:29:37 EST 1994


I've been using dideoxy thermal cycle sequencing, and have had trouble
with "background" in 3 of the 4 lanes- pretty much all the way up and
down the gel. I'm using P32-dCTP, Deep Vent (-), and a plasmid
template. Any info would be appreciated. 

Thanks, Geoff



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