HELP! Method for sequencing 5'ends of cDNA ("RACE")

Edwin Fink fink at
Tue Dec 13 09:56:59 EST 1994

Hello to all,
we want to elucidate the 5'end of a cDNA of which we find only 
clones with incomplete inserts in the library. We have the tissue to 
isolate the mRNA which we want to use for completing the 5'end 
sequence by RACE (rapid amplification of cDNA ends). Has anyone a 
good protocoll? Has anyone experience with the CLONTECH 
5'-Amplifinder RACE kit?
Thanks for your help!        Edwin

Edwin Fink
Department of Clinical Biochemistry
University of Munich
E-mail: fink at

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