Terminator sequencing reactions with Sequitherm?

Muhammad Lodhi lodhi at CSHL.ORG
Tue Dec 13 09:45:08 EST 1994


Has anybody tried using sequitherm polymerase (Epicenter Technologies) in
terminator sequencing reactions instead of Sequenase.  What I am trying is
using ABI's terminator mixture with sequitherm polymerase for cycling
purpose at higher temperatures.  Any comments or experience will be
appreciated.

Muhammad A. Lodhi
Cold Spring Harbor Laboratory





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