Terminator sequencing reactions with Sequitherm?

Muhammad Lodhi lodhi at CSHL.ORG
Tue Dec 13 09:45:08 EST 1994

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Has anybody tried using sequitherm polymerase (Epicenter Technologies) in
terminator sequencing reactions instead of Sequenase.  What I am trying is
using ABI's terminator mixture with sequitherm polymerase for cycling
purpose at higher temperatures.  Any comments or experience will be
appreciated.

Muhammad A. Lodhi
Cold Spring Harbor Laboratory

Previous message: Help! Need vector w/out homol. to pBR322
Next message: HELP! Method for sequencing 5'ends of cDNA ("RACE")
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