concentrating protein lysates

Mary Remington reming at
Tue Dec 13 09:08:45 EST 1994

 We have isolated cellular protein from mammalian cells.  This is total
 cellular protein we are using in a western blot.  We are having a 
 difficult time seeing the protein of interest with our polyclonal Ab.
 Is there a way to concentrate total cellular protein?  Thanks, Mary

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