genome size?

Andrew Cockburn afc at gnv.ifas.ufl.edu
Sat Dec 10 21:29:55 EST 1994


In article <3c0cd8$d1j at mark.ucdavis.edu>, szcooley at chip.ucdavis.edu (Michael Cooley) writes:
> What is the easiest method of determining genome size. I really only need 
> an estimate, I don't want to do CoT analysis is I can avoid it. Seems 
> like I remember some way which involves Southern blots.
> 
> 
> #%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%
> %                                                        #
> #   Dr. Michael Cooley         Practice Random Kindness  %
> %   mbcooley at ucdavis.edu    and Senseless Acts of Beauty #
> #                                                        %
> %#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#

The easiest way is also the most accurate: cytofluorescence.  You stain
cells with a fluorescent dye and measure the fluorescence in an 
appropriate microscope.

Cot analysis is not a reasonable alternative.

Andrew Cockburn



More information about the Methods mailing list