ligation-mediated PCR

Tom Doak tom_doak at hlthsci.med.utah.edu
Tue Dec 13 13:03:42 EST 1994


We are preparing to begin ligation-mediated PCR (LM-PCR), but our reading
indicates considerable confusion about choices of DNA polymerases (with
and without 3', and even 5' exonuclease activities), and about the design
of primers (biotinylated or not, P3 overlapping P2, etc), and a variety
of other details.  We're seeking up-to-date protocols and literature
references.  Any suggestions, comments, hints, or gossip would be very
much appreciated.

Thanks so much,
Tom Doak
SaltLakeCity, Utah
tom_doak at hlthsci.med.utah.edu



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