concentrating protein lysates

[Colin Rasmussen]

On Tue, 13 Dec 1994, Mary Remington wrote:

>  We have isolated cellular protein from mammalian cells.  This is total
>  cellular protein we are using in a western blot.  We are having a 
>  difficult time seeing the protein of interest with our polyclonal Ab.
>  Is there a way to concentrate total cellular protein?  Thanks, Mary
> 
You could try Centricon microconcentrators.