concentrating protein lysates

Colin Rasmussen ras-1 at
Tue Dec 13 11:58:12 EST 1994

On Tue, 13 Dec 1994, Mary Remington wrote:

>  We have isolated cellular protein from mammalian cells.  This is total
>  cellular protein we are using in a western blot.  We are having a 
>  difficult time seeing the protein of interest with our polyclonal Ab.
>  Is there a way to concentrate total cellular protein?  Thanks, Mary
You could try Centricon microconcentrators.

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