concentrating protein lysates
ras-1 at bones.biochem.ualberta.ca
Tue Dec 13 11:58:12 EST 1994
On Tue, 13 Dec 1994, Mary Remington wrote:
> We have isolated cellular protein from mammalian cells. This is total
> cellular protein we are using in a western blot. We are having a
> difficult time seeing the protein of interest with our polyclonal Ab.
> Is there a way to concentrate total cellular protein? Thanks, Mary
You could try Centricon microconcentrators.
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