On density of DNA

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Wed Dec 14 17:00:53 EST 1994


 In article <1994Dec10.213646.5941 at tower>
 afc at gnv.ifas.ufl.edu (Andrew Cockburn) writes:

>> I assume ethidium bromide increases the density of DNA. Is that is true,
>> does anybody know what the density of DNA should be in the presence and in 
>> the absence of EtBr ?.

: Roughly 1.5 g/ml with EtBr, 1.7 g/ml without EtBr.  It will vary according
: to lots of factors, such as G+C, supercoiling, EtBr concentration, etc.
: Note EtBr decreases density of DNA.

Just a strange idea....has anyone tried a CsCl/SYBR Green I plasmid prep?

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