T7 DNA pol for SD mutagenesis

Chuck Hauser chauser at acpub.duke.edu
Wed Dec 14 16:26:39 EST 1994


I have heard that some supplier's T7 DNA polymerase will not work in
Kunkle mutagenesis.  I know that the 3' exo is necessary, and thus
sequenase does not work, but rumor is that some suppliers holoenzyme is
inefficient at repairing.  Does anyone have experience with one which they
know will work?
                  thanks, chuck



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