Transcritional Start Site
library at nimr.mrc.ac.uk
Wed Dec 14 14:47:37 EST 1994
>: determining the transcriptional start site of a gene.
Depending on the amount of RNA transcribed, you could (if there's a lot)
sequence off the end of it using a kinased oligo speciifc to your RNA, RT
and ddNTPs, and if you chase with TdT you can reveal the 5'nucleotide,
or (if there's not a lot of your specific RNA produced)
you could try Clontech's 5' kit (which is just MAGIC) to get clones specific
to the 5'end of your transcript.
More information about the Methods