Transcritional Start Site

a-library library at nimr.mrc.ac.uk
Wed Dec 14 14:47:37 EST 1994


>:  determining the transcriptional start site of a gene.

Depending on the amount of RNA transcribed, you could (if there's a lot)
 sequence off the end of it using a kinased oligo speciifc to your RNA, RT
 and ddNTPs, and if you chase with TdT you can reveal the 5'nucleotide,
 or (if there's not a lot of your specific RNA produced)
 you could try Clontech's 5' kit (which is just MAGIC) to get clones specific
to the 5'end of your transcript.

Matthew 



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