Protein Storage

[Seth M Fisher]

On 13 Dec 1994 12:42:44 -0800, 
Gene Holowachuk  <geneh at USA.NET> wrote:

>Can anyone suggest some guidelines for the storage of highly purified
>recombinantly produced proteins. We have purified a couple of cytokines
>produced in E. coli, about 1 mg/l. What are the best ways for short term
>and long term storage - 50% glycerol, lyophilized, -70 oC, etc. We have
>found loss of activity when stored at 4 oC for longer than 1 week. Any
>suggestions will be appreciated.


A lot of excellent advice has been given already. This is just a caution to 
stay away from -20oC freezers 
if they are frost-free. These freezers actually heat up to defrost and do not 
provide a stable temperature.