Seth M Fisher
Seth_M_Fisher%notes at sb.com
Thu Dec 15 02:19:06 EST 1994
On 13 Dec 1994 12:42:44 -0800,
Gene Holowachuk <geneh at USA.NET> wrote:
>Can anyone suggest some guidelines for the storage of highly purified
>recombinantly produced proteins. We have purified a couple of cytokines
>produced in E. coli, about 1 mg/l. What are the best ways for short term
>and long term storage - 50% glycerol, lyophilized, -70 oC, etc. We have
>found loss of activity when stored at 4 oC for longer than 1 week. Any
>suggestions will be appreciated.
A lot of excellent advice has been given already. This is just a caution to
stay away from -20oC freezers
if they are frost-free. These freezers actually heat up to defrost and do not
provide a stable temperature.
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