Ditto for the usenet

bcdlee at hkpcc.hkp.hk bcdlee at hkpcc.hkp.hk
Mon Dec 12 22:52:22 EST 1994


In article <Rosenwasserlab-051294165831 at 198.243.63.162>, Rosenwasserlab at njc.org (Calvin and Hobbbes) writes:
> Please respond to the usenet also.
> 
> Thanks in advance.
> 
> JS
> 
> In article <3bqbph$7et at mserv1.dl.ac.uk>, christian a geltinger
> <geltinger at gsf.de> wrote:
> 
>> 
>> 
>> Dear netters,
>> 
>> unexpectedly at the end of the year we have some money left (you believe that 
>> ?). So we decided to buy a blotter.
>>  Does somebody have experiences either bad or good with electro and vacuum 
>> blotters to do southern blots mainly? Fragments to resolve will be in the range 
>> of up to 25 kb. Any recomendations and tips will be highly appreciated. 
>> 
>> Please respond directly to the e-mail address
>> 
>> uj23116 at sunmail.lrz-muenchen.de
>> 
>> not to the newsgroup itself ! (We haven+t subscribed yet)
>> 
>> Dr. Horst Schmieger
>> Institut fuer Genetik der LMU Muenchen
>> Tel.: ++49/89/ 1791 9816
>> Fax.: ++49/89/ 1791 9820
>> e-mail: uj23116 at sunmail.lrz-muenchen.de
I have used electro-blotters and vacu-blotters. But, the best reproducible
results are from using alkali capillary blotting onto HYbon N+ membranes.
That is for DNA only. I do think that electro-blotters and vacuum-blotters
are a waste of money. Spend on something else.

Daniel Lee



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