Screening for persence of restriction system

Tom Warnick warnick at microbio.umass.edu
Thu Dec 15 12:03:50 EST 1994


I have been trying to transform a clostridium via electroporation. It works 
fine as long as the DNA is from the same species, but doesn't work at all 
using plasmids extracted from Bacillus; so I suspect that the clostridium has 
a resrtiction nuclease system of some sort. Can anyone recomend a protocol or 
a reference for screening for the presence of a restriction system and perhaps 
also for further characterizing a restriction system?   Thanks,Tom Warnick



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