Competent DH10b/p3's?

T. J. Murphy medtjm at bimcore.emory.edu
Thu Dec 15 17:39:40 EST 1994


Does anyone have a sure-fire protocol specifically for making
chemically competent ecoli ***DH10b/p3 cells***???  We have lots of
competent cell protocols but have very little success with 
these in THIS SPECIFIC ECOLI STRAIN.  BRL seems to have no 
trouble doing this, but of course, they're keeping it a 
secret.  Thanks in advance.


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TJ Murphy
Asst. Professor	      
Dept of Pharmacology
Emory University School of Medicine   	    





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