Competent DH10b/p3's?
T. J. Murphy
medtjm at bimcore.emory.edu
Thu Dec 15 17:39:40 EST 1994
Does anyone have a sure-fire protocol specifically for making
chemically competent ecoli ***DH10b/p3 cells***??? We have lots of
competent cell protocols but have very little success with
these in THIS SPECIFIC ECOLI STRAIN. BRL seems to have no
trouble doing this, but of course, they're keeping it a
secret. Thanks in advance.
---
TJ Murphy
Asst. Professor
Dept of Pharmacology
Emory University School of Medicine
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