TriZOL reagent for RNA isolation?
mtkloth at students.wisc.edu
Thu Dec 15 20:52:47 EST 1994
hardy at mighty.fccc.edu (Richard R. Hardy) wrote:
> Has anyone used the Gibco/BRL TriZOL reagent for RNA isolation from small
> numbers of cells? Opinions pro or con? Favorite technique for isolating
> RNA from small numbers (<100) of cells? Thanks in advance.
> R. Hardy
> Member, Institute for Cancer Research,
> Fox Chase Cancer Center, Philadelphia, PA
> (215) 728-2463
I havn't used Gibco's brand but have used Stratagene's which I believe
is very similar with good results. For extraction from small number of
cells, I typically get very good results. It is also very fast.
On a related note, I just ran accross an article in Biotechniques (1993,
vol 15 I think) that compares a few of the different brands of RNA extraction
kits. This article promotes a kit which claims to allow for RNA, DNA and
protein extraction from the same samples with fairly good results.
RNA could be extracted in 1 hour and DNA & protein in about 3 hours.
I hope this is helpful.
University of Wisconsin
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