Dissolving Qiagen purified DNA

Carsten E. Stidsen ces at novo.dk
Thu Dec 15 08:30:47 EST 1994


In article <3cnede$p83 at mark.ucdavis.edu>, dobates at ucdavis.edu says...
>
>I seem to be having problems getting the DNA pellet from Qiagen to
>dissolve completely in TE. I always seem to end up with some
>precipitate still not in soution even if I heat it up to 65C. The DNA
>seems to be clean (260:280 >1.8) I get a good yield (300-500ug per
>100ml LB), and it works effectively for transfections, but has this ppt
>in it. I get a reduction in transfection efficiency if it's freeze
>thawed over a long period of time (>3months), but that seems to be the
>only problem. However, I'm using this DNA to perfuse through a
>micropipette and the ppt keeps blocking the pipette. Even when I spin
>it I sometimes get blockages. Any suggestions on how to get it to
>dissolve completely?
>
>Thanks
>
>------------------------------------------------------------------------
>-
>Dave Bates PhD                                     Tel: 916 752-7081
>Postdoctoral Researcher                            Fax: 916 758-2554 
>Dept of Human Physiology                         email:
>dobates at ucdavis.edu
>University of California at Davis                drink: anything
>Davis, California 95616, USA            No I don't have a sense of
>humour 
>                                                now buy me a beer      
>------------------------------------------------------------------------
>----       
Hi Dave:
Did you check the pH of your TE? It should be 8, but old solutions of TE 
tend to be closer to neutral.
-- 
Carsten E. Stidsen, Ph.D.
Novo Nordisk A/S
Novo Alle 1CS.36
DK-2880 Bagsvaerd
Phone: +45 4442 2578
Fax:     +45 4498 5007




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