Tricine buffer for PCR

Jasper Saris jasper%ruly46.leidenuniv.nl
Fri Dec 16 03:08:18 EST 1994


Michael Cooley (szcooley at chip.ucdavis.edu) wrote:
: I hear tell of a buffer for PCR based on Tricine. It's supposed to hold a 
: higher pH at the elevated temperatures of the PCR. Has anyone tried this 
: and does anyone know the recipe for making it?


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Hoi Michael,

We use TRIS.HCL at pH8.4, pH8.9 or pH10 on roomtemperature. Most PCR sets 
will work in all three buffers. Often the pH10 variant performs better 
for smeary PCR's. The idea behind this is a higher annealing 
(and elongation?) specificity of the several "Taq" polymerases we use.
Normal is 50mM KCl, 10mM TRIS, 0.5-2.5mM MgCl.

I hope this is helpfull information,

Jasper Saris




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