Ribonuclease Protection Assay Controls ?

Jeffrey Joseph Martino jjm3 at sawasdee.cc.columbia.edu
Sat Dec 17 01:57:43 EST 1994


OK here it goes: Doing Ribonuclease Protection Assays on total RNA
                 from various mouse cells/tissues (Northern no signal,
                 and initial RPAs - no signal)

  - actin controls give a good signal.  

Actin isn't too rare.  Want to put a detection limit number on that
RPA negative result.

I thought adding known quantities of an RNA back to sample as a control
would be OK to check detection limit, but I promised to keep an eye out
for an endogenous alternative:

  Any ideas for a good (not tissue-specific) low level message (with
  probes available) for a control?


Thanks for any and all comments.





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