Ribonuclease Protection Assay Controls ?
Jeffrey Joseph Martino
jjm3 at sawasdee.cc.columbia.edu
Sat Dec 17 01:57:43 EST 1994
OK here it goes: Doing Ribonuclease Protection Assays on total RNA
from various mouse cells/tissues (Northern no signal,
and initial RPAs - no signal)
- actin controls give a good signal.
Actin isn't too rare. Want to put a detection limit number on that
RPA negative result.
I thought adding known quantities of an RNA back to sample as a control
would be OK to check detection limit, but I promised to keep an eye out
for an endogenous alternative:
Any ideas for a good (not tissue-specific) low level message (with
probes available) for a control?
Thanks for any and all comments.
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