Storage of purified proteins

Andreas Buhr Buhr at pki.unibe.ch
Fri Dec 16 12:05:48 EST 1994


 
> In article <9412132042.AA12297 at usa.net>, geneh at USA.NET (Gene Holowachuk) writes:
> > Can anyone suggest some guidelines for the storage of highly purified
> > recombinantly produced proteins. We have purified a couple of cytokines
> > produced in E. coli, about 1 mg/l. What are the best ways for short term
> > and long term storage - 50% glycerol, lyophilized, -70 oC, etc. We have
> > found loss of activity when stored at 4 oC for longer than 1 week. Any
> > suggestions will be appreciated.
> > Cheers :-))
> > 
> > ===========================================================================
> > **  Gene Holowachuk                |  Voice: 607/547-3937                **
> > **  Research Institute             |  Fax  : 607/547-3061 (attn Gene H)  **
> > **  Mary Imogene Bassett Hospital  |  email: geneh at research.mibh.org     **
> > **  One Atwell Road                |       : geneh at usa.net               *
> > **  Cooperstown, NY 13326          |       : geneh at cscns.com             **> > ===========================================================================
 > 
>> 
 
Surprisingly nobody mentioned that there are kryosensitive proteins
(denature by low temperature). Therefore, try also room temperate after
addition of 5 mM NaN3 (microbial growth) and EDTA (proteases).

For literature about protein storage:


R. Scopes "Protein purification" pp 198 ff. Academic Press (1983)

van den Berg, L. and Rose, D. Arch. Biochem. Biophys. 81, 319-329 (1959)

Orii, Y. and Morita, M. J. Biochem. (Tokyo) 81, 163-168 (1977)



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