HELP! Extraction of periplasmic proteins.
Alexander V Kravchuk
akravchu at magnus.acs.ohio-state.edu
Mon Dec 19 13:48:28 EST 1994
Sorry if this is the wrong place to ask the question below!
What is a good method of extraction of periplasmic proteins,
overexpressed in E.coli? The method I am using now (osmotic shock)
does not look very efficient: either I get a lot of intracellular
proteins in the extract, or barely get any protein at all if I try
to do it more gently.
If you know better method and/or can direct me to one, please
reply via e-mail.
Thank you very much in advance!
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