precise quantificatin of genomic DNA from different sources

Michael Cooley szcooley at dale.ucdavis.edu
Mon Dec 19 20:27:31 EST 1994


Brian C. Freeman (bfreeman at casbah.acns.nwu.edu) wrote:
: Need Help: I have problem in quantification of genomic DNA from different
: cell lines. The DNA samples have been treated with RNase A,
: phenol-chloroform, and the OD260/280 are higher than 1.8. Loading the same
: amount of DNA(based on OD260 measuring)  from different samples in agarose
: gel, however, gives a significantly different fluorecent intensity. Should
: I trust the result from OD measuring or from agarose gel?  


Trust the agarose gel. What you need is a fluorimeter. OD measurements 
are often wrong due to other contaminants.


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